Table 2 Select elongation-related probe sets showing altered regulation in the Li ₁ mutant*

Probe ID

Annotation

3DPA

12DPA

16DPA

Heterologous functions

Ref.

Ghi.798.1.S1_s_at

1-aminocyclopropane-1-carboxylate oxidase

2.45

10.95

5.08

GhACO3, ethylene biosynthesis, elongation related

[55]

GhiAffx.16665.1.S1_s_at

1-aminocyclopropane-1-carboxylate oxidase homolog 4-like

1.48

2.87

2.54

no homology known

Ghi.8264.1.S1_s_at

brassinosteroid-regulated protein bru1

0.81

4.11

6.99

homology to xyloglucan endotransglcosylase (AT4G14130.1), expression correlates with elongation inhibition

[56]

Ghi.5860.1.S1_s_at

fasciclin-like arabinogalactan protein

1.10

56.47

0.16

EST from fiber, homology to G. hirs. fasciclin-like arabinogalactan protein 3 (FLA3). AtFLA3 overexpression leads to defective elongation in stamen.

[57]

GhiAffx.36662.1.S1_s_at

r2r3-myb transcription

1.60

4.25

6.40

At4g37260, MYB73. Highly responsive to ethylene, ABA. Downregulated in fl mutant elonagtion phase.

[58, 59]

GhiAffx.60562.1.S1_at

ethylene-responsive transcription factor wri1

0.79

0.31

0.26

wri1-1 mutants demonstrate defective elongation of hypocotyl.

[60]

Ghi.2039.2.S1_s_at

sucrose synthase sus1

35.38

1.60

1.38

GhSus1 isoform C, targeted to cell wall during secondary cell wall synthesis.

[61]

Ghi.7911.1.S1_x_at

xyloglucan endotransglucosylase hydrolase

0.42

9.24

8.22

TCH4, dwarf A. thaliana mutants have reduced expression, elongation related.

[62]

GhiAffx.10621.1.A1_s_at

pollen ole e 1 allergen and extensin family protein

1.21

32.53

0.67

Uncharacterized EST

Genes down-regulated in the Li ₁ mutant relative to its near isogenic wild type line

Ghi.8025.1.S1_s_at

1-aminocyclopropane-1-carboxylate oxidase

1.37

0.36

0.32

ACO4, ethylene biosynthesis, induced in ovule culture by fatty acid synthesis, increasing ethylene production and root elongation.

[55, 63]

Ghi.6502.1.S1_at

1-aminocyclopropane-1-carboxylate oxidase

1.13

0.63

0.14

EST, significant homology to ACO proteins, but not identical to ACO1-4

Ghi.5451.1.S1_at

1-aminocyclopropane-1-carboxylate synthase

0.78

0.47

0.88

Highest homology with GhACS 3/6

GhiAffx.12577.1.S1_at

gibberellin 20-oxidase

0.95

0.34

0.15

gibberellin synthesis, stimulated by Auxin treatment in A. thaliana, which also causes hypocotyl cell elongation. Increased expression in transgenic cotton causes increased fiber length.

[64–66]

Ghi.8087.1.S1_s_at

myb-like transcription factor 3

1.32

0.18

0.16

GhMYB3, contains gibberellin responsive GLABROUS1, which promotes trichome formation in A. thaliana.

[67, 68]

Ghi.10822.1.S1_at

xyloglucan endotransglucosylase hydrolase

1.10

0.41

0.52

GhXTH2, high homology to coding region of GhXTH1 which produces longer fibers when over-expressed in transgenic cotton.

[69]

GraAffx.28354.1.S1_s_at

rho gtpase activation protein

0.52

0.48

0.78

High homology to ROP1, tip-localized GTPase responsible for cell elongation and polarity.

[70]