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Figure 7 | BMC Genomics

Figure 7

From: Genetic heterogeneity revealed by sequence analysis of Mycobacterium tuberculosis isolates from extra-pulmonary tuberculosis patients

Figure 7

Analysis of PCR products derived from Primer pairs (Panels A-H) detailed in Table3using agarose gel electrophoresis. Panels A, B and C represent specific coordinates of IS element insertion in the isolates, whereas panels D and E represent those from where IS element has been translocated in the isolates in comparison to the reference strain H37Rv. Panel H (IS6110 internal primers) confirms the presence of IS6110 element in all the strains. Co-ordinates for IS element insertion/deletion and expected amplicon size in the respective strains are detailed in Table 3. Lane 1: Negative control; Lane 2: 100-3000 bp DNA Ladder; Lane 3–8: H37Rv DNA (Lane 3) and Triton extracts of isolates (Lane 4:AC74; Lane 5:AC544; Lane 6:LN8; Lane 7: F85; Lane 8: F99). [Lane 2: DNA Ladder; with IS element; : without IS element].

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