Figure 1

Monoallelic expression of cubilin in Cubn^{+/del exon 1–6;EGFP}mice leads to reduced albumin binding to brush borders of cubilin-deficient renal proximal tubules cells. A shows a confocal micrograph of a section of wildtype mouse kidney labeled with anti-cubilin IgG (red) and the nuclear stain, Draq5. B shows a confocal image from a section of a Cubn^{+/del exon 1–6;EGFP} mouse kidney labeled with anti-cubilin and Draq5. Asterisks indicate proximal tubules displaying relatively low levels of anti-cubilin immunolabeling. C and D are merged images of anti-cubilin immunolabeling (red) and anti-EGFP immunolabeling (green) and nuclear staining (blue). D is a digitally zoomed in view of the boxed area in C. E and G show confocal images of anti-albumin labeled sections from wildtype (E) and Cubn^{+/del exon 1–6;EGFP} (F) mouse kidneys. F and H show images of the sections shown in E and G merged with anti-EGFP labeling. G, glomerulus.