Figure 10

TSA-mediated increase in cubilin expression involves increases in both PPAR transcription and activation. A, qPCR analysis of PPARα mRNA levels in NRK cells transfected with PPARα expression construct or a control vector. Asterisk in A indicates that differences between mRNA levels in control and PPARα were significant at p<0.05. B, qPCR analysis of cubilin mRNA levels in NRK cells transfected with PPARα expression construct or a control vector and treated for 24 h with either PPARα agonist, Wy14643 (100 μM) , TSA (100 nM) or TSA (100 nM) plus PPARα agonist (100 μM). Asterisks indicate that differences between mRNA levels in vehicle control and drug treatment groups were significant at p<0.05. Pound signs indicate that differences between mRNA levels in control vector and PPARα vector transfected cells with the same treatments were significant at p<0.05.