Figure 5

5Aza and TSA elicit increases in cubilin expression in NRK and Caco-2 cells. A-D, qPCR analysis of cubilin and megalin mRNA expression in RNA isolated from NRK and Caco-2 cells cultured for 60 h with three medium changes and 84 h with four medium changes, respectively, each containing the indicated doses of 5Aza. E-H, qPCR analysis of cubilin and megalin mRNA expression in RNA isolated from cells cultured for 24 h with the indicated doses of TSA. Cubilin and megalin mRNA expression data were normalized to GAPDH levels. Asterisks indicate that differences between mRNA levels in vehicle control and drug treatment groups were significant at p<0.05.