From: Combined methylation mapping of 5mC and 5hmC during early embryonic stages in bovine
 | Blastocyst embryos | Elongation embryos | All | ||||
---|---|---|---|---|---|---|---|
Method | Me-RDA | HMe-RDA | HELP cocktail | Me-RDA | HMe-RDA | HELP cocktail | Â |
Msp/Hpa | FspBI/BfaI | Msp/Hpa | FspBI/BfaI | ||||
Raw reads from library | 313 330 | 179 105 | 242 864 | 208 963 | 94 961 | 243 874 | 1 283 097 |
Cleaned readsa | 281 619 | 72 855 | 234 149 | 192 163 | 71 459 | 218 874 | 1 071 119 |
Validated readsb | N/A | N/A | 46 835 | N/A | N/A | 50 462 | N/A |
Consensus sequencesc | 33 123 | 29 416 | 31 604 | 25 616 | 24 963 | 41 784 | 186 506 |
Sequences with genomic alignmentsd | 28 941 | 25 360 | 25 519 | 18 944 | 14 978 | 37 759 | 151 501 |
Sequences with unique alignmente | 1890 | 11 772 | 14 712 | 1317 | 8858 | 30 587 | 69 136 |
Putative methylated restriction sitesf | 3634 | 21 352 | 18 810 | 2584 | 15 714 | 39 367 | 101 461 |