Comparison of gene expression in OP and CP libraries. A) Mapping and assembly statistics for OP and CP replicates. QC = quality control (see Methods); FPKM = fragments (i.e. reads) per kilobase exon per million mapped reads. For genes assembled using Tuxedo pipeline, B) Venn diagram showing genes with an FPKM value of 1 in OP and CP libraries. C) Log2 (Fold Change) distribution (CP/OP) is shown as determined by edgeR.