Figure 6

Up-regulation of cell wall and cuticle genes is associated with epidermal impermeability of wheat plants to the neutral red dye in infested resistant plants. The names of the genes and their putative functions are given on the left part of the figure. Fold changes and I/C ratio in transcript abundance are shown in the middle of the figure along with the numbers of probe sets that detected the changes. I/C ratio were defined as in Additional file 4: Figure S1. Results of neutral red staining for epidermal cell permeability are shown on the right. For the neutral red staining panels, NU1 and NU2 are two representatives of un-infested, susceptible Newton plants, whereas NI1 and NI2 are two Newton plants infested with Hessian fly. No staining was observed in the un-infested plants, but very strong staining was observed in infested Newton plants. MU1 and MU2 are two representatives of un-infested Molly plants, whereas MI1 and MI2 are two infested Molly plants. No staining was observed in the un-infested Molly plants, and no or very weak staining was observed in infested Molly plants.