Predicted new members of the exported gene family phist in several Plasmodium species, including rodent parasites. (A) Left: Heat map of pairwise BLAST sequence similarities between all proteins of the PHIST cluster. Shades of red indicate degree of similarity (red = high similarity, white = no similarity). Right: Compressed PHIST dendrogram extracted from the hierarchical tree. Annotated phist gene family members indicated in blue. Putative new gene family members shown in red (full-length proteins) or black (annotated pseudogenes or gene fragments). Note the putative new phist gene family members in rodent parasites at the bottom. Black triangles indicate collapsed subtrees with numbers of genes contained in this subtree specified in parentheses. (B) Top: Pfam family PF09687 (PRESAN domain) sequence logo highlighting conserved key residues of the phist gene family. Bottom: Corresponding conserved blocks extracted from a T-coffee multiple sequence alignment of selected putative new PHIST members. Note that these putative new gene family members have most key residues of the PRESAN domain conserved, suggesting that they are likely true members of the diverse phist gene family.