Figure 4
GR and AP-1 motifs co-occurrence in the genome and GR peaks. (A) Enrichment of split 8-mers (N-NNN---NNN-N) vs. all continuous 8-mers in the GR ChIP-seq peaks. Red dots: the canonical GR motif and 1-bp variants (mismatch in red), Blue dots: the 8-mers with AP-1 motif.(B) The co-occurrence of canonical GR and AP-1 motifs at distances up to 900-bps in masked mouse genome (blue) and GR peaks (red). The bin size is 50-bps with 1-bp sliding window. (C) The co-occurrence of canonical GR and AP-1 motifs within a nucleosome range (<150-bps) is statistically significant in both the genome (p=3×10-53) and GR peaks (p=4×10-162). (D) Same as (C) but for GR and AP-1 motifs separated by 150-bps to 900-bps. Their co-occurrences in the genome are close to expected occurrences (p=0.08) but enriched in GR peaks (p=4×10-49). (E) The co-occurrence of canonical GR and AP-1 motifs within a nucleosome range (<150-bps) is statistically significant in both the DHS regions (p=2×10-7) and GR peaks (p=1×10-179). (F) Same as (E) but for GR and AP-1 motifs separated by 150-bps to 900-bps. Their co-occurrences in the DHS regions are close to expected occurrences (p=0.77) but enriched in GR peaks (p=3×10-12). (G) Number of GR motifs with 0, 1, or 2 or more AP-1 motifs within 150-bps and y-axis represents percent in the GR peaks. The * denotes the statistical significance (p<1×10-10) of co-occurring GR and AP-1 motifs being bound by GR protein compared to GR motifs without an AP-1 motif. (H) Percent of canonical or 1-bp variants of GR motifs with (blue) or without (red) canonical AP-1 motifs within 150-bps in the GR peaks. Motifs are presented by their enrichment in the GR peaks.