Figure 5

In AtT-20 pituitary cells, GR binds regions with high INOS and co-occurs with E-box motifs. (A) Average INOS near (±750-bps) canonical GR motifs (G-ACA---TGT-C); 390 bound and 26,786 unbound GR motifs in AtT-20 pituitary cells are shown. (B) GR binds preferentially to the GR motifs with higher INOS in AtT-20 cell line. See Figure 1C. 1.44% of all GR sites are bound by GR (black dashed line). (C) AP-1 motifs are not enriched in GR peaks in AtT-20 cells. (D) Number of GR motifs with 0, 1, or 2 or more E-box 7-mer (CAGCTGT) within 150-bps in the genome and percent bound by GR are presented along y-axis. The * denotes the statistical significance (p<0.01) of co-occurring GR and E-Box motifs being bound compared to GR motifs without an E-Box motif. The red dashed line in panels C-D is the average binding ratio of the GR motif. (E) The co-occurrence (9,651) of a canonical GR and E-Box motif at various distances up to 900-bps in the masked mouse genome and fraction in AtT-20 GR ChIP-seq peaks. The bin size used is 50-bps with 1-bp sliding window.