The duration of gastrin treatment affects global gene expression and molecular responses involved in ER stress and anti-apoptosis

Table 1 Enrichment analysis

	P-values	Differentially expressed genes
Enrichment by GeneGo pathway maps
Apoptosis and survival_Endoplasmic reticulum stress response pathway	6,910E-05	ATF-4, GRP78, C/EBP zeta, IP3R1, HERP, DNAJC3, ERP5
Reproduction_GnRH signalling	7,392E-05	PER1, JunB, ATF-3, EGR1, IP3 receptor, Dynamin-1, HDAC5, c-Fos
DNA damage_ATM / ATR regulation of G2 / M checkpoint	1,262E-04	Wee1, Cyclin B, GADD45 alpha, Cyclin A, CDK1 (p34)
Development_Growth hormone signalling via PI3K/AKT and MAPK cascades	1,485E-04	JunB, C/EBP zeta, 4E-BP1, EGR1, C/EBPbeta, c-Fos
Development_Hedgehog and PTH signalling pathways in bone and cartilage development	6,180E-04	EGR1, Smoothened, Ihh, Cyclin A, c-Fos
Enrichment by GeneGo process networks
DNA damage_Checkpoint	1,639E-05	CIA/ASF1, PCNA, Wee1, Cyclin B, GADD45 alpha, Cyclin B2, ATF-3, Cyclin A1, 14-3-3 theta, Cyclin A, CDK1 (p34), CDK6, 14-3-3
Cell cycle_Mitosis	5,376E-05	Tubulin beta, Kid, PARD6A, Wee1, Cyclin B, CSE1L, Cyclin B2, TTK, CAS-L, MKLP1, Dynamin-1, Cyclin A, CDK1 (p34), PARD6, Dynamin
Protein folding_Response to unfolded proteins	3,657E-04	ATF-4, ERp44, GRP78, HSP70, Calreticulin, HERP, DNAJC3, SELS

Canonical pathways and networks most significantly associated with the 403 genes differentially expressed (p<0.05) in transient versus sustained gastrin treatment shown in the heat map in Figure 1B and in Additional file 1. The 5 pathways and 3 networks with lowest p-values are shown as determined by the MetaCore tool of GeneGo package [15]. Enrichment analysis by GO process is found in Additional file 3: Tables S2 and S3.

ISSN: 1471-2164