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Table 3 STRs validation result of homologous and heterologous primer pairs of water buffalo

From: In silico mining of putative microsatellite markers from whole genome sequence of water buffalo (Bubalus bubalis) and development of first BuffSatDB

 

Heterologous

Homologous

 

ISAG–FAO recommended STRs from cattle

ISAG–FAO recommended STRs from buffalo

Nagarajan et al, monomorphic STRs from buffalo

Nagarajan et al, polymorphic STR loci from buffalo

Total no. of primer pairs reported

30

30

7

107

No. of positive primers (Forward)

7 (23.33%)

7 (23.33%)

2 (28.57%)

49 (45.79%)

No. of positive primers (Reverse)

11 (36.67%)

8 (26.67%)

4 (57.14%)

37 (34.58%)

No. of positive primers (common to both forward and reverse)

3 (10.00%)

4 (13.33%)

2(28.57%)

26 (24.30%)