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Figure 7 | BMC Genomics

Figure 7

From: Genomic distribution of SINEs in Entamoeba histolytica strains: implication for genotyping

Figure 7

Strain identification in xenic cultures based on locus 13, 17 and 19: PCR was performed using genomic DNA of 16 different xenic cultures of E. histolytica for each locus, as described in Figure 6. PCR reactions were resolved on a 1 % agarose gel and subjected to Southern blotting with the locus specific probes 13 (Panel A), 17 (Panel B), or 19 (Panel C). Samples which did not give a product at locus 13 were amplified using an alternate reverse primer 13.1 R instead of 13.2 R followed by Southern blotting and hybridization with a locus specific probe. The expected size of the amplicon with each primer set is mentioned in the table below each locus panel.

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