Figure 6

In situ hybridization analysis of patched - 2 and pax7 expression in the regenerating blastemas. Patched-2 (A) or pax7 (C) signals (blue) using respective anti-sense probes are shown in the distal tip of regenerating limbs. No signal was detected in the negative-control sections using respective sense probes of patched-2 (B) and pax7 (D) on serially sectioned slides. The insets in the left upper are the enlarged photos of respective areas indicated by dotted lines at blastema. Asterisks indicate apical epithelial cap covering the blastema. Scale bars indicate 200 μm.