Method of identifying splicing-regulated MREs in a given tissue. Step (I) is describing the data source used to derive information about alternative splicing events of mRNA, tissue and histology, and MRE. Step (II) is schematically depicting the above three data types. Step (III) is integrating these three types of information and specifies the EST configuration (see “Methods” for detailed description). Step (IV) is establishing the two-way contingency Table used for further analysis. Step (V) is performing a statistical test to identify splicing-related MREs that have increased in a given tissue.