Genomic rearrangements in the evolved clone BW4005 sampled from a glucose-limited chemostat. (a) RFLP-IS3 of EcoRV-digested genomic DNA of BW2952 and BW4005.2 . A fifth hybridizing fragment (arrow) with stronger hybridization intensity was detected in BW4005.2. (b,c) Maps showing the genomic regions corresponding to the 10.6 and 7.9 kb EcoRV IS3-hybridizing fragments, including the sequences adjacent to IS3 in BW2952 (b) and BW4005.2 (c). Genomic coordinates (given in parentheses) and gene orientations (horizontal boxes) are based on the genome sequence of BW2952 . Vertical arrows indicate EcoRV restriction sites, and horizontal arrows show locations of primers used to identify the junction of the duplicated region (Table 2). The triangle shows the location of the 2668-bp deletion in BW4005.2. (d) PFGE of XbaI-digested genomic DNA of BW2952 and BW4005.2. The additional bands in BW4005.2 compared to BW2952 are shown by arrows. (e) RFLP-IS3 and PFGE profiles of six clones (BW4005.C1 to BW4005.C6) derived from the initial glycerol stock of BW4005. The sizes of relevant bands are indicated on the left.