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Table 3 Phenotypic traits of ancestral, evolved and reconstructed E. coli strains

From: A case of adaptation through a mutation in a tandem duplication during experimental evolution in Escherichia coli

Strains Description Yielda Acetate productionb Fitness (Sh-1)c
50:50 1:99
BW2952 Ancestor 1.00 - 1.00 1.00
BW4005.2 Derivative of evolved isolate BW4005 0.89 ± 0.02 + −0.04 ± 0.01 0.06 ± 0.01
BW4005.C2 Derivative of BW4005 (DO)d 0.74 ± 0.08 + −0.09 ± 0.01 ND
BW4005.C3 Derivative of BW4005 (WT)d 0.98 ± 0.03 - −0.02 ± 0.01 ND
BW4005.C6 Derivative of BW4005 (HD)d 0.83 ± 0.05 + −0.04 ± 0.01 ND
BW6029 BW2952 yegS::bla 0.85± 0.03 + −0.02 ± 0.01 0.09± 0.04
BW6030 BW2952 yegR::bla 1.01 ± 0.02 - 0.01 ± 0.01 −0.01 ± 0.01
BW6031 BW2952 ∆ogrK-yegS-yegR::bla 0.88 ± 0.02 + −0.05 ± 0.01 0.11± 0.04
  1. aGrowth yields are shown relative to the ancestral strain BW2952 and were obtained from 24-hour glucose-limited chemostats by measuring the optical density of the cultures at 600 nm. Values are from three independent measurements.
  2. bAcetate production was tested with 24-hour chemostat cultures. +, acetate present at 2–5 mg l-1; -, below the detection limit (< 0.15 mg l-1). Data were from at least three independent biological repeats with less than 10% standard errors.
  3. cCompeting strains were mixed at 50:50 or 1:99 ratios after individual acclimation in the chemostat evolution conditions. Changes in the proportions of the competitors after 24 hours were used to calculate fitness values. Data presented are mean ± SEM from at least two independent experiments. ND, not determined.
  4. dSymbols are identical to the legend of Figure 3.