Detailed view of Fse I and T-tailed adapters used during preparation of DG template for sequencing. (A.) FseI and (B.) T-tailed adapter nucleotide sequences are shown. Primer binding sites for the first and second PCR and single-read sequencing reactions are indicated. Unique sequences within the adapter for the first and final PCR steps are denoted in green and blue, respectively. X’s denote the location of the 4 bp index sequence. The full complement of index sequences may be found in Additional file 4.