Figure 7

DG template preparation workflow. Genomic DNA is digested with FseI. Index adapters are ligated to the FseI ends. The DNA fragments are randomly sheared, followed by size selection on agarose gels. DNA fragments of a selected size are end-repaired. A T-tailed adapter is ligated to the repaired ends of the DNA. PCR is carried out with a biotinylated oligonucleotide primer complementary to the Index adapter. DNA fragments labelled with biotin are captured via magnetic beads. The purified DNA fragments are amplified by PCR with Illumina Primers. Amplification products are sequenced on the Illumina GAIIx sequencer. The colored regions in the DNA fragments correspond to the colored regions in the detailed views of the Index and T-tailed adapters in Figure 6.