Table 1 Restriction enzymes used for digital genotyping
From: Digital genotyping of sorghum – a diverse plant species with a large repeat-rich genome
Restriction enzyme | Recognition sequence | No. of potential sequences RE sites x 2 ( In silico) | DG sequences unique, 33 bp ( In silico) | DG sequences unique, 33 bp (Sequenced >3x) |
|---|---|---|---|---|
Fse I | GGCCGG∨CC | 46,068 | 24,670 | 22,272 |
Ngo MIV | G∨CCGGC | 329,032 | 190,382 | ~155,000 |
Hpa II | C∨CGG | 2,872,516 | ~1,540,000 | ~572,000 |