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Table 1 Restriction enzymes used for digital genotyping

From: Digital genotyping of sorghum – a diverse plant species with a large repeat-rich genome

Restriction enzyme Recognition sequence No. of potential sequences RE sites x 2 ( In silico) DG sequences unique, 33 bp ( In silico) DG sequences unique, 33 bp (Sequenced >3x)
Fse I GGCCGGCC 46,068 24,670 22,272
Ngo MIV GCCGGC 329,032 190,382 ~155,000
Hpa II CCGG 2,872,516 ~1,540,000 ~572,000