Transcriptome analysis of porcine M. semimembranosus divergent in intramuscular fat as a consequence of dietary protein restriction

Table 2 Genes selected for realtime quantitative PCR (qPCR) validation of differential expression observed on the microarray

Gene category	Annotation probe ID	Gene symbol	Function	Microarray		qPCR
				FC*^#	P-value	FC*^#	P-value
Differentially expressed genes	Ssc.27307.1.S1_at	MPHOSPH6	Protein binding, RNA degradation	1.208	0.0009	1.413	0.006
	Ssc.24599.1.S1_at	UBE2CBP	Ligase activity	1.179	0.010	1.293	0.005
	Ssc.9340.1.A1_at	LTBR	Apoptosis	2.178	0.018	1.908	0.031
	Ssc.16159.1.S1_at	SCD	Synthesis of unsaturated fatty acids	2.374	0.011	6.2	0.066
	Ssc.17286.1.A1_at	BTG2	Protein binding	1.763	0.012	2.418	0.015
	Ssc.28320.1.S1_at	CBX5	Chromatin binding, enzyme binding	1.357	5.86E-07	1.214	0.091
	Ssc.17345.1.S1_at	ANGPTL4	Enzyme inhibitor activity, receptor binding	−2.204	7.3133E-06	−2.355	0.003
	Ssc.3020.1.A1_at	IQGAP2	Actin binding, cytoskeleton	−1.415	1.6622E-05	−1.358	0.098
	Ssc.26308.1.S1_at	PROCR-like	Receptor activity	−1.991	0.001	−2.461	0.013
	Ssc.27508.1.A1_at	SATB2	Protein coding, transcription factor	−1.409	7.1385E-05	−1.358	0.10
Non-changing genes	Ssc.11026.1.S1_at	RAP1	Cell adhesion and cell junction formation	1.000	0.98	1.267	0.16
	Ssc.14406.1.A1_at	MRPS6	Encodes ribosomal protein	1.001	0.96	1.241	0.22
	Ssc.1480.1.S1_at	PSMD1	Substrate recognition and binding	1.002	0.90	1.286	0.15

*FC: Fold change.
^#Positive fold changes indicate that the low protein (LP) diet resulted in higher expression than the high protein (HP) diet and the number represents [expression LP/ expression HP]. Negative fold changes indicate that the LP diet resulted in lower expression than the HP diet and the number represents {−[expression HP / expression LP]}.
Gene expression changes (fold changes) and P-values determined by microarray and qPCR are shown.

ISSN: 1471-2164