Figure 5

Leishmania braziliensis morphology and α-tubulin RNA accumulation after heat shock treatment. Promastigotes cultures were incubated at 26 (A) or 35°C with 5% CO₂ during 30 min (B), 1 hour (C), 2 hours (D) and 4 hours (E). Total RNA was extracted from each time point, and RNA aliquots of 8 μg each were analyzed by Northern blotting (F). The blots were hybridized with the α-tubulin ORF probe and ethidium bromide rRNA staining was used as normalizing signal.