Table 9 Characteristics of genes necessary for spermatogenesis whose repression following heat shock in spermatocytes correlates with HSF1 binding
Gene symbol (full name) | Changes in expression (SLR) | HSF1 binding (AB1-AB0) | Function | ||||
|---|---|---|---|---|---|---|---|
| Â | SC_38 vs C | SC_43 vs C | HEP_43 vs C | SC_38 | SC_43 | HEP_43 | Â |
Dnaja1 (DnaJ (Hsp40) homolog, subfamily A, member 1) | −1.09 | −0.07 | 1.17 | 34.4 | 69.9 | 40.5 | Cochaperone of HSP70s in protein folding and mitochondrial protein import. Its loss in mice led to severe defects in spermatogenesis [31]. |
Celf1 (CUGBP, Elav-like family member 1) | −1.07 | −0.01 | 0.19 | 35.1 | 48.3 | 24.5 | Post-transcriptional regulation: pre-mRNA alternative splicing, mRNA translation and stability. Required for completion of spermatogenesis [32]. |
Spo11 (sporulation protein, meiosis-specific, SPO11 homolog (S. cerevisiae)) | −1.19 | 0.07 | −0.03 | 33.0 | 53.3 | - | A type II like topoisomerase; required for meiotic recombination [33, 34]. |
Piwil2 (piwi-like homolog 2 (Drosophila)) | −1.20 | −0.22 | −0.04 | 58.3 | 69.8 | - | Participates in the repression of transposable elements in spermatogenic cells; involved in translation regulation [35, 36]. |
Tdrd1 (tudor domain containing 1) | −1.57 | −0.40 | 0.42 | 23.8 | 48.1 | - | Participates in the repression of transposable elements in spermatogenic cells [37]. |
Mov10l1 (Moloney leukemia virus 10-like 1, homolog (mouse)) | −1.07 | −0.32 | 0.23 | 60.8 | 94.5 | - | Putative RNA helicase, essential for silencing retrotransposons in the mouse male germline [38, 39]. |