Systems infection biology: a compartmentalized immune network of pig spleen challenged with Haemophilus parasuis

Table 3 Primer sequences, melting temperatures and product sizes for twelve immunogenes in the qPCR analysis

Gene symbol	Primer sequence (5'-3')	Target size (bp)	Tm (°C)
ADAM17	Sense: TGGCAGACAACATCGTGGG	106	59
ADAM17	Anti-sense: GGGCTTGATGATGCGAACG	106	59
ARG2	Sense: GCATTCCGCCCCGAAG	233	65
ARG2	Anti-sense:CCACTGAGCGAGGATTCACT	233	65
C1R	Sense: TGTGGACCTGGATGAATGT	101	59
C1R	Anti-sense: AATAGCCGCCGATGTAGTT	101	59
CFB	Sense: CATTCATGATATTCGGGACCT	92	59
CFB	Anti-sense: GCCCAACCCCAAACACGTA	92	59
CD1D	Sense: GTGGCTCCTCTACGACATCT	88	55
CD1D	Anti-sense: TTCAGGCTTCACTTGCTTCTC	88	55
BCL6	Sense: TTGTCATAGTGGTGAGCCGAGAG	168	65
BCL6	Anti-sense: TGAAGTCCAGGAGGATGCAGAAC	168	65
ADM	Sense: CGCTACCGCCAGAGTATGAACA	121	59
ADM	Anti-sense: CGTCCTTGTCTTTGTCCGTGAAC	121	59
IFIT1	Sense: AATTAGCCACAGGTCATT	144	55
IFIT1	Anti-sense: ATTCCATACACAACACTCT	144	55
ASHA2	Sense: AATCTCCTGCTATATTAGAAG	126	55
ASHA2	Anti-sense: AGTTCCTACATCTCCATT	126	55
PAK1	Sense: CACTTCCTATTACTCCAACT	113	59
PAK1	Anti-sense: CTTCTTCTTCTGCTTCTCA	113	59
ST6GAL1	Sense: GACATCATTCAAGAAATCTC	164	59
ST6GAL1	Anti-sense: AAGAACTTCTGGTAGTAGTA	164	59
RPL32 ^a	Sense: CGGAAGTTTCTGGTACACAATGTAA	94	55-65
RPL32 ^a	Anti-sense: TGGAAGAGACGTTGTGAGCAA	94	55-65

ISSN: 1471-2164