FISH of fosmids on cucumber somatic and pachytene chromosomes 1, 3–5. a1-a4 FISH of fosmids on somatic chromosomes. Adjacent probes were labeled with different fluorochromes and hybridized together to test the order and position. b1-b4 Cucumber chromosomes at the pachytene stage were probed by a set of fosmid clones together with the Type III satellite repeat. c1-c4 Two straightened cucumber pachytene chromosomes. The left chromosome was straightened from the image shown in Figures b1-b4. d1-d4 The chromosomes in Figures c1-c4 were converted into black-white image. Bars, 5 μm.