Platyhelminth DNMTs are novel monophyletic members of the DNMT2 DNA methyltransferase family. Phylogenetic relationships were inferred from a concatenated multiple sequence alignment (MUSCLE v3.8) of platyhelminth and representative eukaryotic DNMT sequences (NCBI accession numbers are listed in Methods), using Bayesian (implemented in MrBayes v3.1.2) and Maximum Likelihood (implemented in MEGA v5.05) approaches. The figure shows the Bayesian consensus tree illustrated by TreeView, where branch lengths (indicated by scale bar) represent distance between taxa. Only nodes with Bayesian posterior probability support values greater than 0.5 are shown. Node labels within parentheses represent percentage bootstrap support values from Maximum Likelihood analysis (1000 bootstrap replicates performed), while those outside parentheses represent Bayesian posterior probability support values. Platyhelminth sequence names are as listed in Figure 1. Notations Am, Mm, Bm, Ci, Cq, Ct, Cg, Lg and Hr correspond to A. mellifera, M. musculus, B. mori, C. intestinalis, C. quinquefasciatus, C. teleta, C. gigas, L. gigantea and H. robusta sequences respectively. The platyhelminth DNMT2 clade is indicated by a dashed box.