Figure 3

Platyhelminth MBD candidates share sequence conservation across both Pfam methyl-CpG binding domain (PF01429) and the C-terminal domain of methyl-CpG binding protein 2 and 3 (PF14048) motifs. Amino acid sequence alignment of the methyl-binding domain (PF01429) and the C-terminal domain of methyl-CpG binding protein 2 and 3 (PF14048) of MBD homologs from S. mansoni (Sm), S. japonicum (Sj), P. westermani (Pw), C. sinensis (Cs), O. viverrini (Ov), F. hepatica (Fh), E. multilocularis (Em), E. granulosus (Eg), T. solium (Ts), H. microstoma (Hm), S. mediterranea (Smd) and M. lignano (Ml) with M. musculus MBD2 and MBD3 proteins and the P. pacificus MBD protein (NCBI accession numbers are listed in the Methods). Amino acid residues with known functional importance are indicated with an asterisk in upper line. Numbers at the beginning of each line represent amino acid positions and at each position the most conserved residues are further shaded in black, semi-conserved residues are highlighted grey and non-conserved amino acids are kept white. The row labelled 'consensus' represents the Pfam consensus sequence of each domain where conserved amino acids (50–79%) are indicated by lower-case and highly conserved residues (> 80%) by upper-case letters. Missing amino acid residues, not present in the truncated PwMBD and MlMBD candidates, are indicated by a ‘N/A’.