Figure 4
From: Cytosine methylation is a conserved epigenetic feature found throughout the phylum Platyhelminthes
Platyhelminth MBD candidates are novel members of the invertebrate specific MBD2/3 clade of methyl-CpG binding domain containing proteins. Phylogenetic relationships were inferred from a multiple sequence alignment (MUSCLE v3.8) of platyhelminth and representative eukaryotic MBD domains (NCBI accession numbers are listed in the Methods) using Bayesian (implemented in MrBayes v3.1.2) and Maximum Likelihood (implemented in MEGA v5.05) approaches. The figure shows the Bayesian consensus tree illustrated by TreeView, where branch lengths (indicated by scale bar) represent distance between taxa; only nodes with Bayesian posterior probability support values greater than 0.5 are shown. Node labels within parentheses represent percentage bootstrap support values from Maximum Likelihood analysis (1000 bootstrap replicates performed), while those outside represent Bayesian posterior probability support values. Notations Hp, Bm, Xl, Xt, Mm, Ct, Cg, Lg and Hr correspond to H. pulcherrimus, B. mori, X. laevis, X. tropicalis, M. musculus, C. teleta, C. gigas, L. gigantea and H. robusta sequences. The platyhelminth MBD2/3 clade is indicated by a dashed box.