From: Assessing De Novo transcriptome assembly metrics for consistency and utility
Metric | Trend by | Consistent over | Trend by | Consistent over | Fully consistent |
---|---|---|---|---|---|
sequencing depth | sequencing depths | read length | read lengths | metric | |
Contig count |
| ✓ |
| ✓ | ✗ |
% of reads used in contigs |
| ✓ |
| ✓ | ✓ |
BP in contigs |
| ✓ |
| ✓ | ✓ |
% BP in contigs |
| ✓ |
| ✓ | ✓ |
Average contig coverage |
| ✗ |
| ✗ | ✗ |
Average unigene coverage |
| ✓ |
| ✓ | ✓ |
Contig read count COV |
| ✓ |
| ✓ | ✗ |
Unigene read count COV |
| ✓ |
| ✓ | ✗ |
Average contig length |
| ✗ |
| ✗ | ✗ |
Average unigene length |
| ✓ |
| ✓ | ✓ |
Contig N50 length |
| ✗ |
| ✗ | ✗ |
Unigene N50 length |
| ✓ |
| ✓ | ✓ |
Unique annotations in singletons |
| ✓ |
| ✓ | ✗ |
Unique annotations in contigs |
| ✓ |
| ✓ | ✓ |
Unique annotations in unigenes |
| ✗ |
| ✓ | ✗ |
Average contig OHR |
| ✗ |
| ✗ | ✗ |
Average unigene OHR |
| ✓ |
| ✓ | ✓ |
Contig RBH count |
| ✓ |
| ✓ | ✓ |
Unigene RBH count |
| ✓ |
| ✓ | ✓ |
% of Annotated contigs with RBHs |
| ✗ |
| ✗ | ✗ |
% of Annotated unigenes with RBHs |
| ✓ |
| ✗ | ✗ |
Average contig CF |
| ✓ |
| ✓ | ✓ |
Average unigene CF |
| ✗ |
| ✗ | ✗ |
Unique reverse annotations in contigs |
| ✓ |
| ✓ | ✓ |
Unique reverse annotations in unigenes |
| ✓ |
| ✓ | ✓ |