The modified library protocol recapitulates known regions of enrichment in H3K27me3 ChIP. Qualitative depiction of tag density and cluster enrichment. ng: data generated from 5–50 ng ChIP DNA by modENCODE. pg1, pg2: biological replicates of data generated from ~100 pg ChIP DNA for this study. The chromatin in the ng experiment is fragmented by sonication, while the chromatin in the pg experiment is fragmented by micrococcal nuclease. A: Clusters of region of enrichment on the entirety of chr3R with a significance threshold of Z-score =3 and enrichment of 2-fold or more. y-axis is 0–3 for all samples. B: Input subtracted tag density and corresponding regions of enrichment (based on M-values) in ~1000 kb of chr3R. Y-axis is 0–250 for tag density samples and 0–3 for cluster samples. chr3R: chromosome 3R. Selected genes noted in italics.