Figure 2

Impact of read depth on the ability to discriminate between homeologs in polyploid genomes. Read-depth schematic of Locus_5955, homologous to MPBQ-methyltransferase (VTE3). Vertical color bars inside plot represent the proportion of each putative distinct base (SNP) at a particular position (A) area with lower read coverage. (B) and (C) areas with higher coverage. Magnified areas show detail of the reads at each putative polymorphism site, for each one of the three transcript isoforms: iso_1 (Locus_5955_1/3), iso_2 (Locus_5955_2/3) and iso_3 (Locus_5955_3/3). Underneath each pile-up of reads appears the base called by Oases. The outlined graph represented in the figure corresponds to the second isoform of the locus (Locus_5955_2/3).