MicroRNAome profiling in benign and malignant neurofibromatosis type 1-associated nerve sheath tumors: evidences of PTEN pathway alterations in early NF1 tumorigenesis

Table 1 List of the significantly deregulated miRNAs in plexiform neurofibromas relative to dermal neurofibromas

miRNAs	Functionally verified gene target(s)	Location	Host gene	Dermal neurofibromas (n = 9)	Plexiform neurofibromas (n = 41)	Fold change^a	P ^b	Peripheral nerves (n = 2)^c
Significantly upregulated miRNAs in plexiform neurofibromas relative to dermal neurofibromas
miR-486-3p	PTEN[10]	8p11.2	ANK1	1.3 [0.03-12.8]^d	9.3 [0.02-198.0]	7.41	0.002	1.4 [0.90-1.8]
miR-185		22q11.2		2.8 [0.10-23.6]	16.0 [0.04-97.5]	5.67	0.003	8.4 [3.1-13.8]
miR-362-5p		Xp11.2	CLCN5	4.8 [0.09-72.6]	18.5 [0.03-312.0]	3.90	0.008	21.3 [7.2-35.4]
miR-370	MAP3K8[11]	14q32.2		13.8 [5.4-33.0]	28.7 [0.08-232.6]	2.08	0.008	11.5 [10.8-12.1]
miR-491-5p		9p21.3		12.8 [2.0-22.1]	22.6 [0.02-115.3]	1.76	0.014	27.6 [23.8-31.3]
miR-143	KRAS[12]	5q32		185.0 [68.1-411.0]	437.7 [3.3-3156.7]	2.37	0.016	426.1 [339.9-512.4]
miR-193a-5p		17q11.2		2.0 [0.58-13.5]	16.9 [0.02-168.9]	8.57	0.017	11.3 [8,0-14.5]
miR-483-5p		11p15.5	IGF2	7.4 [0.27-24.2]	19.7 [0.02-326.9]	2.67	0.020	112.3 [38.7-185.8]
miR-181a	KRAS[13] and ATM[14]	1q32.1 and 9q33.3	NR6A1 (9q33.3)	8.2 [0.71-84.3]	36.3 [0.02-231.0]	4.44	0.022	10.7 [5.7-15.7]
miR-145	RREB1[15]	5q32		436.3 [259.8-2572.9]	1792.9 [22.9-14808.8]	4.11	0.024	830.3 [688.1-972.4]
Significantly down-regulated miRNA in plexiform neurofibromas relative to dermal neurofibromas
miR-203		14q32.3		36.2 [1.2-10966.7]	4.8 [0.05-2943.7]	0.13	0.010	2.6 [2.3-2.8]

^aMedian expression in plexiform neurofibromas/Median expression in dermal neurofibromas.
^bMann-Whitney’s U Test.
^cNon tumorigenic controls.
^dMedian [range] of miRNAs levels.

ISSN: 1471-2164