Genome-wide identification of binding sites for NAC and YABBY transcription factors and co-regulated genes during soybean seedling development by ChIP-Seq and RNA-Seq

Table 2 Synthetic peptides used to develop antibody against YABBY and NAC transcription factor

TF name	Gene model	Amino acid position	Sequence of synthetic peptides
			Used for antibody production
NAC	Glyma12g04380.1^*	13-26	VRTGGKGTMRRKKK
	Glyma11g12170.1
	Glyma11g12180.1^* Glyma01g18780.1
YABBY	Glyma13g22620.1	99-112	TEERVVNRPPEKRQ
	Glyma17g12200.1

All the antibodies are produced by GenScript Corporation. They used James and Wolf (JW) prediction algorithm to design synthetic peptides for the production of antibody against YABBY and NAC transcription factor.
^* Used for single amino acid difference between Glyma12g04380.1 and synthetic peptide at amino acid position 21 where Valine was replaced with Methionine (M).

ISSN: 1471-2164