Figure 8

Identification and tissue distribution of novel genes. Using the novel splice junctions (green bars) and coverage data (red), transcripts with 3 or more novel exons located in intergenic regions were identified as putative novel genes (transcript models in black). A) An example of a five exon gene (NG78) with multiple isoforms that does not contain an ORF. B) An example of a six exon gene (NG106) that contains an ORF and is believed to be protein coding. C) Primers were designed in the putative terminal exon regions of the novel genes, and subject to RT-PCR and Sanger sequencing for validation of expression. NG78 was found in the original 3 retinal samples, an independent retinal sample (retina 4), as well as in the brain, liver, and muscle. NG106 was not found in the liver and muscle.