Table 2 Overview of the sequencing and assembly
| Â | Cold-treated | Control | Total |
|---|---|---|---|
Total Raw Reads | 71,441,910 | 73,279,028 | Â |
Total Clean Reads | 65,075,656 | 67,287,120 | Â |
Total Clean Nucleotides (nt) | 5,856,809,040 | 6,055,840,800 | Â |
Q20 percentage | 97.39% | 97.60% | Â |
N percentage | 0.00% | 0.00% | Â |
GC percentage | 45.87% | 45.40% | Â |
Contig | |||
Total Number | 145,000 | 148,797 | Â |
Total Length(nt) | 45,723,903 | 51,308,749 | Â |
Mean Length(nt) | 315 | 345 | Â |
N50 | 521 | 619 | Â |
Unigene | |||
Total Number | 76,000 | 84,583 | 82,795 |
Total Length(nt) | 48,956,203 | 57,108,594 | 67,554,337 |
Mean Length(nt) | 644 | 675 | 816 |
N50 | 1122 | 1191 | 1343 |
Total Consensus Sequences | 76,000 | 84,583 | 82,795 |
Distinct Clusters | 1,831 | 2,043 | 40,988 |
Distinct Singletons | 74,169 | 82,540 | 41,807 |