A. Diagram of the bioinformatic pipeline for the A. carolinensis reannotation. B. Venn diagram illustrating the sources of data for the A. carolinensis reannotation. Ab initio, algorithm based gene predictions using Augustus and SNAP[26–28]. RefSeq, alignments of zebrafish, Xenopus frog, chicken, mouse, and human protein and available vertebrate transcripts to the Anocar2.0 genome assembly. NCBI/Ensembl, combined data of A. carolinensis genome annotations from NCBI ref_Anocar2.0 and Ensembl Build 65. RNA-Seq, transcriptomic data from analysis of 14 adult and embryonic tissues.