Genome reannotation of the lizard Anolis carolinensis based on 14 adult and embryonic deep transcriptomes

BMC Genomics

Table 2 Comparison of ASU, NCBI and Ensembl gene annotations of the A. carolinensis genome

Overview	ASU	NCBI	Ensembl
Annotated genes	22,962	15,645	17,792
Annotated transcript isoforms	59,373	16,533	18,939
Annotated isoforms/gene	2.59	1.06	1.06
Annotated Transcripts
All transcript isoforms	59,373	16,533	18,939
Transcripts with start & stop codons	53,401	14,667	4,170
Transcripts missing start or stop codon	5,972	1,866	14,769
Single exon transcripts	2,070	983	364
Transcript N50 length	5,355	2,364	2,037
Average coding sequence length	1,964	1,701	1,531
Exons
Total number of exons	229,204	156,742	174,545
Exons with start with codon	29,677	13,512	5,971
Exons without start or stop codon	168,367	128,486	158,935
Exons with stop codon	29,727	13,779	9,278
Exons/annotated transcript	12.05	10.11	9.62
Average exon length	170	170	160
Total exon length	38,902,806	26,658,387	27,910,718
3' UTR
Total transcripts with 3'UTR	34,926	5,861	0
Average length of transcripts with 3'UTR	1,168	456	0
Total 3'UTR sequence length	40,798,794	2,674,388	0
5' UTR
Total transcripts with 5'UTR	46,782	6,168	0
Average length of transcripts with 5'UTR	244	86	0
Total 5'UTR sequence length	11,422,626	527,454	0
Introns
Total number of introns	192,418	141,362	155,949
Average intron length	4,525	4,463	2,553
Total intron sequence length	870,771,088	630,937,171	398,124,572

ISSN: 1471-2164