qPCR reassessment for seven ESTs after treatments with JH, JH + SHE and SHE from the follow-up bioassay. A) Shows a positive correlation of qPCR results from follow-up bioassays against microarray fold change results for the six JH influenced ESTs (Spearman rank correlation, Rs = 0.89, df = 5, P = 0.02). The empty circle represents an EST that was downregulated by both JH and SHE. B-H) Show expression profiles for three JH upregulated ESTs (B, C and D), two JH downregulated ESTs (E, F and H), one SHE downregulated EST (G) and one SHE upregulated EST (H). The expression level is presented as 2-ΔΔCT, calculated from qPCR cycle threshold value. The microarray fold change values are provided in the parentheses for each EST. FL644436 (G) was downregulated by both JH (fold change: 0.543) and SHE (fold change: 0.811).