Number of SNVs detected by the three exome sequencing replicate experiments. In total, 194,929 SNVs were identified in all the three replicates, whereas 1202 and 772 were found in two and one replicates, respectively. Of the 192,614 SNVs present in the SPRET/EiJ genome reference sequences, 99.4%, 99.8% and 99.9% could be identified in at least three, two and one replicates, respectively. A total of 4505 SNVs identified in at least one replicates were not found in the SPRET/EiJ genome reference sequences. 3559 and 436 of them could be detected in three and two replicates, respectively.