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Figure 6 | BMC Genomics

Figure 6

From: Suppression of genetic recombination in the pseudoautosomal region and at subtelomeres in mice with a hypomorphic Spo11 allele

Figure 6

DSB repair in Gal4BD-Spo11 spermatocytes is incomplete. Chromosomal spreads from earlier (zygotene) and later (pachytene) stage spermatocytes were immunostained with antibodies to γH2AX (red) and SCP3 (green). Note the disappearance of γH2AX staining at homologously synapsed autosomes in later stage wild type and Spo11Gal/Gal spermatocytes consistent with proper repair of DSBs. γH2AX persist in Spo11Gal/- spermatocytes and on asynapsed chromosomes in Spo11Gal/Gal spermatocytes. Sex body (white arrow) is evident in wild type, but not Gal4BD-Spo11 spermatocytes.

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