Suppression of genetic recombination in the pseudoautosomal region and at subtelomeres in mice with a hypomorphic Spo11 allele

Table 1 Gal4BD-SPO11 directs a population of hotspots to the sites with Gal4 consensus motif

Sample	Sequenced ssDNA tags, ×10⁶	Identified hotspots	Hotspots with Gal4 consensus		Hotspots present in the wild type mice
Wild type	49.4	18,313	416	(2.3%)	18,313	(100%)
Hop2 ^−/−	3.4	8,221	204	(2.5%)	8,036	(97.7%)
Spo11 ^Gal/Gal	17.5	10,313	224	(2.2%)	10,086	(97.8%)
Spo11 ^Gal/Gal Hop2 ^−/−	46.3	13,445	546	(4.1%)	12,730	(94.7%)

The genotype of mouse strains is listed in the left column. Sequenced ssDNA tags indicate the number of ssDNA-derived fragments (e.g., those derived from ssDNA tails of DSBs) that have been sequenced for this analysis. The number of hotspots identified in each mouse strain is also indicated (column 3) and the overlap of these hotspots with the hotspots found in the wild type mice (“wild type” sample) is specified (column 5). The number of hotspots that contain Gal4 consensus motif (column 4, left) and the percentage of Gal4-containing hotspots in the total number of hotspots identified in the corresponding samples (column 4, right) are shown to demonstrate the increase in the Gal4-containing hotspots in the Spo11^Gal/GalHop2^−/− mice (see text for more explanation).

ISSN: 1471-2164