Skip to main content
Figure 5 | BMC Genomics

Figure 5

From: RNAi phenotypes are influenced by the genetic background of the injected strain

Figure 5

Similar strength of RNAi in black and SB strains. Parental RNAi (pupal injection and analysis of the offspring) is shown for Tc-dll (A) and Tc-giant (Tc-gt) (B). Larval RNAi was done for Tc-wg (C) and Tc-six3 (D). In all panels, the gray bars represent results for SB strain while black bars represent the black strain. (A,C,D) The phenotypes were grouped into four classes of phenotypic strength represented in the pictures below the panel (Level 1: mildest phenotype; Level 4: strongest phenotype). To calculate the mean phenotypic strength, the phenotype classes were given arbitrary values of 1 to 4 points point for level 1 to level 4. (B) In case of Tc-gt, the number of missing abdominal segments is used for grouping the phenotypes into classes. In all cases, we find a similar distribution of phenotypic strength. Hence, none of the strains appears to be generally more amenable to RNAi than the other in pupal or larval RNAi. Cx coxa, Tr trochanter, Fe femur, Ti tibia, Ptc pretarsal claw.

Back to article page