Figure 6
From: RNAi phenotypes are influenced by the genetic background of the injected strain
Amino acid exchanges in the cargo binding domain of Importin α1 between the strains. Shown is a section of an alignment of Importin α proteins of different species and strains of Tribolium including the 2nd, 7th and 8th ARM domains. GA2 is the sequenced strain. Black boxes represent identical amino acids, gray boxes denote sites with a conservation of about 90% and horizontal lines indicate the conserved ARM domains involved in cargo binding identified in S. cerevisiae SRP1 [48]. Red frames indicate two sites in which the amino acid sequence of the Tribolium strains differ. Both sites are within the region known to be responsible for cargo binding. Note, that other metazoans show N or S at the respective position within the ARM2 domain (see text for details).