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Table 2 Expressed sequence tag (EST) data for the putative diatom G protein-coupled receptors (GPCRs)

From: Identification of G protein-coupled receptor signaling pathway proteins in marine diatoms using comparative genomics

Sequence Accession no. (JGI/NCBI) Length (aa) % EST coverage (≥98% identity) No. of EST sequences EST coverage region (aa) (≥98% identity) Culture treatment Reference
P. multiseries        
GPCR1 240179 906 45 1 499-906 Phosphate starvation 1JGI
GPCR2 117604 1003 17 1 616-819 Domoic acid-producing conditions 2
GPCR3 179685 521 No hits N/A N/A N/A N/A
GPCR4 288475 461 No hits N/A N/A N/A N/A
GPCR5 293132 887 No hits N/A N/A N/A N/A
F. cylindrus        
GPCR1 242057 911 No hits N/A N/A N/A N/A
GPCR2 241103 1020 No hits N/A N/A N/A N/A
GPCR3 236496 1102 No hits N/A N/A N/A N/A
GPCR4 266398 899 30 2 32-305 Osmotic stress, pooled RNA (5 treatments) 3
GPCR5 253555 257 No hits N/A N/A N/A N/A
P. tricornutum        
GPCR1A 219114538 688 100 64 1-688 16 different treatments [20]
GPCR1B 219117625 626 100 64 1-619 16 different treatments [20]
GPCR2 219114407 799 69 8 1-552 16 different treatments [20]
GPCR3 219113755 1111 86 22 1-228/378-1111 16 different treatments [20]
GPCR4 219113191 1012 74 18 1-125/366-986 16 different treatments [20]
T. pseudonana        [25]
GPCR1 224005915 627 No hits N/A N/A 5Upregulated during silaffin-like response [25]
GPCR2 224006404 846 26 1 570-792 Iron-limited cells 4JGI
  1. N/A, not applicable.
  2. 1http://genome.jgi-psf.org/Psemu1/Psemu1.home.html.
  3. 2Boissonneault KR, Bates SS, Milton S, Pelletier J, Housman DE: Gene discovery and expression profiling in the toxin (domoic acid)-producing marine diatom Pseudo-nitzschia multiseries (Bacillariophyceae) using cDNA microarrays, unpublished.
  4. 3Krell A, Gloeckner G: Analysis of an osmotic stress induced cDNA library of the psychrophilic diatom Fragilariopsis cylindrus, unpublished.
  5. 4http://genome.jgi-psf.org/Thaps3/Thaps3.home.html.
  6. 5T. pseudonana GPCR1 had no corresponding EST data but the sequence was found to be upregulated during transcriptome profiling under silicon starvation conditions.