Identification of G protein-coupled receptor signaling pathway proteins in marine diatoms using comparative genomics

Table 2 Expressed sequence tag (EST) data for the putative diatom G protein-coupled receptors (GPCRs)

Sequence	Accession no. (JGI/NCBI)	Length (aa)	% EST coverage (≥98% identity)	No. of EST sequences	EST coverage region (aa) (≥98% identity)	Culture treatment	Reference
P. multiseries
GPCR1	240179	906	45	1	499-906	Phosphate starvation	¹JGI
GPCR2	117604	1003	17	1	616-819	Domoic acid-producing conditions	²
GPCR3	179685	521	No hits	N/A	N/A	N/A	N/A
GPCR4	288475	461	No hits	N/A	N/A	N/A	N/A
GPCR5	293132	887	No hits	N/A	N/A	N/A	N/A
F. cylindrus
GPCR1	242057	911	No hits	N/A	N/A	N/A	N/A
GPCR2	241103	1020	No hits	N/A	N/A	N/A	N/A
GPCR3	236496	1102	No hits	N/A	N/A	N/A	N/A
GPCR4	266398	899	30	2	32-305	Osmotic stress, pooled RNA (5 treatments)	³
GPCR5	253555	257	No hits	N/A	N/A	N/A	N/A
P. tricornutum
GPCR1A	219114538	688	100	64	1-688	16 different treatments	[20]
GPCR1B	219117625	626	100	64	1-619	16 different treatments	[20]
GPCR2	219114407	799	69	8	1-552	16 different treatments	[20]
GPCR3	219113755	1111	86	22	1-228/378-1111	16 different treatments	[20]
GPCR4	219113191	1012	74	18	1-125/366-986	16 different treatments	[20]
T. pseudonana							[25]
GPCR1	224005915	627	No hits	N/A	N/A	⁵Upregulated during silaffin-like response	[25]
GPCR2	224006404	846	26	1	570-792	Iron-limited cells	⁴JGI

N/A, not applicable.
¹http://genome.jgi-psf.org/Psemu1/Psemu1.home.html.
²Boissonneault KR, Bates SS, Milton S, Pelletier J, Housman DE: Gene discovery and expression profiling in the toxin (domoic acid)-producing marine diatom Pseudo-nitzschia multiseries (Bacillariophyceae) using cDNA microarrays, unpublished.
³Krell A, Gloeckner G: Analysis of an osmotic stress induced cDNA library of the psychrophilic diatom Fragilariopsis cylindrus, unpublished.
⁴http://genome.jgi-psf.org/Thaps3/Thaps3.home.html.
⁵T. pseudonana GPCR1 had no corresponding EST data but the sequence was found to be upregulated during transcriptome profiling under silicon starvation conditions.

ISSN: 1471-2164