Figure 7

Experimental validation of an EC in the STMN2 gene by PCR-sequencing. A: EC in STMN2 gene on chromosome 8 of HF087. The enlarged figure shows the position of the PCR amplicon. B: Restriction digest of the PCR amplicon. The undigested amplicon is 1003 bp. Digestion with SwaI restriction enzyme is expected to yield two fragments of 519 bp and 484 bp (based on the location of the EC in the optical map). An addition digestion was performed with NheI enzyme to ensure the correct amplicon was being analyzed. The expected sizes of the NheI fragments are 616 bp and 387 bp. C: Sequence of the PCR amplicon showing the G > A transition that creates a new SwaI cut site (blue triangle).