Figure 4
From: Identification of medium-sized genomic deletions with low coverage, mate-paired restricted tags
A 3290-bp deletion that skips three consecutive restriction sites on chromosome 17. Ditags were used to design a pair of primers to amplify the breakpoint-containing sequences. The results showed bands of different sizes for the control and the test DNA. The breakpoint sequence was identified by direct Sanger sequencing with a micro-insertion observed at the break sites.