Figure 7

Overrepresented motifs upstream of COWPs by subclass. A. Expression profiles of Class I and Class II COWPs. The five COWPs that fall into Class 1 peak at 48 hrs post-infection and then decline. The remaining four Class II COWPs begin rising at 48 hrs and peak at 72 hrs. B1. The upstream regions of each of the Class I COWPs contain five overrepresented motifs that fall into three groups. Upstream regions for each of these genes were mined for overrepresented motifs (see Materials and Methods). Three motifs overrepresented upstream of Class I COWPs are closely related to E2F binding sites. A GAGA-like motif and an ApiAP2 motif identified in P. falciparum (Campbell et al. 2010; here we designate this motif AP2_2) are also overrepresented upstream of Class I COWPs. B2. The upstream regions of each of the Class II COWPs contain five overrepresented motifs. Two motifs are similar to a documented ApiAP2 binding site across apicomplexans. E2F-like and CCAAT-box-like motifs are also overrepresented. The remaining motif is unknown and does not appear related to any of the 25 motifs identified in this study.