Figure 7

Variation in the number of SNPs discovered in the combined assembly with Minor Allele Frequency (MAF) and depth of sequence coverage. Local regression was used to fit a smoothed spline to the raw dataset, comprising 14,574 SNPs identified using the Newbler mapping program, implemented using the ‘locfit’ package in R. The area shaded in red indicates the parameter space associated with the greatest numbers of SNPs discovered.