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Figure 2 | BMC Genomics

Figure 2

From: TGFβ and CCN2/CTGF mediate actin related gene expression by differential E2F1/CREB activation

Figure 2

TGFβ and CCN2 together induce differential gene expression associated with altered functional and canonical pathways and unique transcription factor representation. A, B. Treatment of HMCs with TGFβ, CCN2 or both together induced differential expression of shared and unique subsets of genes. For each condition, n = 3 gene arrays were used with the average determined by GC Robust Multi-array average and the change in gene expression determined using Student’s t-test and Benjamini-Hochberg correction at p < 0.05. C, D. Pathway analysis with Ingenuity IPA software identified the augmentation of functional pathways associated with the cell cycle, cell growth and proliferation and renal hypertrophy/glomerular injury; canonical pathways including signalling via the TGFβ superfamily and cAMP were decreased in activity while increased ERK/MAPK activity was identified. E, F. Co-inertia analysis of the promoters of the genes differentially expressed in response to TGFβ, CCN2 or both together identified TFBSs that were over and under-represented in specific gene subsets at p < 0.05.

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